CpGenome Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.

:Materials Provided

Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.

:Validation

Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only .after the DNA was methylated